Molekularbiologie: Grundlagen, Methoden und Anwendung

It is the aim of this laboratory course to provide basic molecular biology techniques in the first place. Students should experience isolation and manipulation of DNA and RNA in a way that they understand the underlying principles of these fundamental methods. In a second step, students have to learn different techniques used to transfect pro- and eukaryotic cells as these are important methods in biotechnology. Therefore, the course provides the complete scenario from the preparation of DNA up to the functional analysis of different expression plasmids. Third, students will become familiar with viruses, as these biological agents are important "work horses“ in different areas of molecular biology. Working with microbeads to detect nucleic acids and proteins in a multiplex format provides the opportunity for state of the art bioanalytical methods. This part is complemented by genome editing experiments. Students will get in touch with a state of the art complex methodology and get hands-on experience. The seminar has the task of broadening the theoretical basis of the methods used in the practical course. Presentations and enhanced discussions will provide the stage for a fruitful enhancement of molecular biology knowledge. In addition to specialized knowledge (professional competences), the students will train social competences because of being stimulated for contributions and critical discussions. Methodical competences are developed as well because students have to train their practical lab skills and generic competences as they have to solve scientific problems. All these projects require search and understanding of relevant scientific literature, design of experimental protocols, documentation and a critical discussion of results.

• Practical laboratory work-preparation of experiment, performance, discussion, time management (50%)
• Presentation and discussion in the seminar; duration depends on the topic (25%)
• Lab report (including title, abstract, introduction, methods and materials, results, discussion, conclusion, and references; 15-20 pages) (25%)

• iGenetics: A Molecular Approach. Peter J. Russell. ISBN 10: 0805346651
• Molecular Biology of the Gene. James D. Watson, Tania A. Baker, Stephen P. Bell et al. ISBN-13: 9780321507815
• Molecular biotechnology / by Carolyn A. Dehlinger; Jones & Bartlett Learning, ISBN 978-1-284-05783-6
• Variable original publications depending on the project given.
• Protocols and manuals of the manufacturers of reagents and kits used during the lab course are provided as pdf-files using the university intranet. Seminar literature will be provided upon request.
• On the other hand it is part of the seminar that students have to search for appropriate information and to develop different information acquisition strategies.

• Biotechnology (Master of Science, 3 Semester Version): Master 1st year course
• Biotechnology (Master of Science, 4 Semester Version): Master 2nd year course

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