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14122Fakultät 2 – Umwelt und Naturwissenschaften
Biomedizinischer Laborkurs III - Zellkultur
Titel auf Englisch: Biomedical Lab Course III - Cell culture
Lernergebnisse
- After completing the module, the students will be able to present the current literature on a research area in a summarized form. By presenting and discussing their results from the laboratory course they are able to critically question research results. In the practical course, students have to work on sophisticated projects which need competences such as teamwork, organized project planning (project management) and analytical abilities
- Acquire theoretical knowledge and practical experience in basic principles of cell culture techniques. …
Inhalte
- Sterile working technique, media preparation
- Routine maintenance of cell lines: Medium replacement, subculture of adherend and suspension cells, cell counting, cryopreservation, contamination control (microscopical observation, mycoplasma screening)
- Cultivation of cells in monolayer (2D)
- Application of special test systems to monitor proliferation, viability, and toxicity in monolayer cells
- Analyses of morphology of cells in 2D
- Working with different microscopic techniques: Phase contrast microscopy (basis “life cell” analyses), brightfield microscopy, fluorescence microscopy
Voraussetzungen
Verpflichtende Voraussetzungen
Empfohlene Voraussetzungen
Keine
Prüfungsmodalitäten
- Successful completion of the occupational safety instruction, submission of the report on the experiments carried out during the laboratory training (35-45 pages), ungraded
- Written exam 120 min
Angeboten in
- Life Science and International HealthBachelor
Wahlpflichtkomplex: Kein Komplex genannt
| Studiengang | Abschluss | Wahlpflichtkomplex |
|---|---|---|
| Life Science and International Health | Bachelor | Kein Komplex genannt |
Literatur und Materialien
- "Cell Culture for Biologists: A Practical Approach" by D. Rickwood and B.D. Hames - This text offers practical guidance on cell culture techniques, with a focus on sterile working practices.
- further required material will be provided (script for the experiments)
Hinweise
no offer in winter semester 2026/27
Bewertungen von Studierenden
Was Studierende über dieses Modul sagen. Die BTU hat damit nichts zu tun — hier schreiben nur Studierende, und jede Bewertung ist die Meinung einer einzelnen Person.